Transcriptomics

Definition: Deep sequencing of cDNA prepred from total RNA.
See also
mRNA-seq (Deep sequencing of cDNA prepred from mRNA.)
Open reviews
Microarrays, deep sequencing and the true measure of the transcriptome. Malone JH, Oliver B. BMC Biol. 2011 May 31;9:34. PMID: #21627854# [Free]
Paywall Reviews
The diagnostic power of RNA-seq. Nature Reviews Genetics (2017) doi:10.1038/nrg.2017.39.
RNA-Seq and find: entering the RNA deep field. Roberts A, Pachter L. Genome Med. 2011 Nov (...)

Transcriptomics studies often rely on partial reference transcriptomes that fail to capture the full catalogue of transcripts and their variations.
Recent advances in sequencing technologies and assembly algorithms have facilitated the reconstruction of the entire transcriptome by deep RNA sequencing (RNA-seq), even without a reference genome.
However, transcriptome assembly from billions of RNA-seq reads, which are often very short, poses a significant informatics challenge.
Reviews (...)