RNA study

• RNA splicing profiling in tumors
  18 October 2012

  splicing profiling in cancer; splice profiling in cancer; splicing profiles in cancer; splice profiles in cancer;
  Presentation
  "RNA expression and splicing profiling from fine-needle samples of tumors" by Dr Philippe Viehl, IGR, France at the 2012 Biopathology Symposium.

• RNA study from fne needle aspiration
  18 October 2012

  RNA study from fne needle aspirates
  Presentation
  "RNA expression and splicing profiling from fine-needle samples" by Dr Philippe Viehl, IGR, France at the 2012 Biopathology Symposium.

• mRNA amplification after laser microdissection
  1 January 2012

  References
  Amplification of mRNA from laser-microdissected single or clustered cells in formalin-fixed and paraffin-embedded tissues for application in quantitative real-time PCR. Theophile K, Jonigk D, Kreipe H, Bock O. Diagn Mol Pathol. 2008 Jun;17(2):101-6. PMID: #18382351#

• RNA quality assessment
  7 December 2011

  References
  Quality assessment of microarray data in a multicenter study. Archer KJ, Mas VR, O’Brien TR, Pfeiffer R, Lum NL, Fisher RA. Diagn Mol Pathol. 2009 Mar;18(1):34-43. PMID: #19214110#

• RNA preamplification
  7 December 2011

  Molecular signatures of tumors with prognostic and predictive value are now available. However, several technical problems prevent the performing of gene expression profiles in routine diagnostics.
  The highly sensitive fluorescence-based real-time quantitative reverse transcriptase-polymerase chain reaction procedure is able to analyze mRNA levels from formalin-fixed paraffin-embedded (FFPE) tissues, the most commonly available source of tumor samples with well-documented information. (...)

• taqman low-density array
  7 December 2011

  Small amounts of RNA from paraffin-embedded tissues can be successfully amplified without altering the gene expression. (#19704261#)
  TaqMan low-density array technology could be used successfully for the analysis of archival fixed and paraffin-embedded lymphoid tissue. (#19704261#)
  References
  Multiple gene expression analyses in human lymphoid tissues by taqman low-density array using amplified rna isolated from paraffin-embedded samples. Gloghini A, Canal B, Dal Maso L, Carbone A. (...)

• RNA virus detection from FFPE
  7 December 2011

  References
  Real-time quantitative assay for routine testing of HCV RNA in formalin-fixed, paraffin-embedded liver samples. Gruppioni E, Vasuri F, Fiorentino M, Capizzi E, Altimari A, Pirini MG, Grazi GL, Malvi D, Grigioni WF, D’Errico-Grigioni A. Diagn Mol Pathol. 2009 Dec;18(4):232-8. PMID: #19861893#

• single-labeling cDNA microarrays-based gene expression profiling
  7 December 2011

  References
  Automated brain tumor biopsy prediction using single-labeling cDNA microarrays-based gene expression profiling. Castells X, García-Gómez JM, Navarro A, Acebes JJ, Godino O, Boluda S, Barceló A, Robles M, Ariño J, Arús C. Diagn Mol Pathol. 2009 Dec;18(4):206-18. PMID: #19861896#

• RT-PCR from FFPE
  5 December 2011

  References
  Analytical performance of a qRT-PCR assay to detect guanylyl cyclase C in FFPE lymph nodes of patients with colon cancer. Beaulieu M, Desaulniers M, Bertrand N, Deschesnes RG, Beaudry G, Garon G, Haince JF, Houde M, Holzer TJ. Diagn Mol Pathol. 2010 Mar;19(1):20-7. PMID: #20186008#

• qRT-PCR from FFPE
  5 December 2011

  Formalin-fixed, paraffin-embedded lymphoid samples are suitable for Q-RT-PCR when using thoroughly validated reference genes.
  Gene expression analysis of formalin-fixed paraffin-embedded (FFPE) material by quantitative real-time polymerase chain reaction is a valuable tool for the retrospective analysis of clinical samples.
  The degraded nature of RNA from FFPE tissue can lead to variation in detection of gene expression between samples, hence, it is necessary to select reference genes (...)

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