RPAs
Members
| RPA1 | RPA2 | RPA3 |
Function
RECQL3 binds to the 70 kDa subunit of the heterotrimeric, single-stranded DNA binding protein, replication protein A (RPAs). This interaction stimulates the helicase activity of RECQL3.
RPAs are involved in DNA replication, repair and recombination, and can be detected on meiotic prophase chromosomes where it appears to play a role in both homologous synapsis and recombination.
RECQL3 and RPAs also colocalise in meiotic prophase nuclei of mouse spermatocytes. Potentially, RECQL3 and RPAs could work together to unwind various DNA structures to facilitate DNA recombination during meiosis, perhaps through resolving recombination intermediates.
References
Wang, Y.; Putnam, C. D.; Kane, M. F.; Zhang, W.; Edelmann, L.; Russell, R.; Carrion, D. V.; Chin, L.; Kucherlapati, R.; Kolodner, R. D.; Edelmann, W. : Mutation in Rpa1 results in defective DNA double-strand break repair, chromosomal instability and cancer in mice. Nature Genet. 37: 750-755, 2005. PubMed ID : #15965476#
Chaudhuri, J.; Khuong, C.; Alt, F. W. : Replication protein A interacts with AID to promote deamination of somatic hypermutation targets. Nature 430: 992-998, 2004. PubMed ID : #15273694#
Zou, L.; Elledge, S. J. : Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes. Science 300: 1542-1548, 2003. PubMed ID : #12791985#