EM-ISEL

EM-ISEL is used to assess DNA single-strand breaks (SSBs) expressed as number of immunogold particles per microm2 of chromatin at both chromosomal and nuclear DNA levels.

See also

 genotoxicity
 DNA single-strand breaks (SSBs)

References

 Depault F, Cojocaru M, Fortin F, Chakrabarti S, Lemieux N. Genotoxic effects of chromium(VI) and cadmium(II) in human blood lymphocytes using the electron microscopy in situ end-labeling (EM-ISEL) assay. Toxicol In Vitro. 2006 Jun;20(4):513-8. PMID: 16278061

 Assad M, Yahia LH, Rivard CH, Lemieux N. In vitro biocompatibility assessment of a nickel-titanium alloy using electron microscopy in situ end-labeling (EM-ISEL). J Biomed Mater Res. 1998 Jul;41(1):154-61. PMID: 9641635

 Assad M, Lemieux N, Rivard CH. Immunogold electron microscopy in situ end-labeling (EM-ISEL): assay for biomaterial DNA damage detection. Biomed Mater Eng. 1997;7(6):391-400. PMID: 9622107

 Migheli A, Attanasio A, Schiffer D. Ultrastructural detection of DNA strand breaks in apoptotic neural cells by in situ end-labelling techniques. J Pathol. 1995 May;176(1):27-35. PMID: 7542332